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nti βactin  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology nti βactin
    Nti βactin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 46336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nti+%CE%B2actin/%CE%B2-Actin+Antibody/pm39494508-51-62-64
    Average 96 stars, based on 46336 article reviews
    nti βactin - by Bioz Stars, 2026-10
    96/100 stars

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    Staining:

    Article Title: ALS-associated FUS mutation reshapes the RNA and protein composition of stress granules.
    Article Snippet: Protein electrophoresis was performed using 4–15% Mini-PROTEAN TGX Precast acrylamide gel (Bio-Rad), and proteins were transferred to 0.45 μm nitrocellulose membrane, using the TransBlot Turbo S t 5 b i a ( ( F 1 a o p W t ( P F A w C w A b t w p t 1 u I s C d 1 P t a p P f G F 1 T t t w R d P l 3 a w l s i I t D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae942/7874844 by guest on 07 N ovem ber 2024 ystem (Bio-Rad), according to the manufacturer’s instrucions. .. Membranes were stained with Ponceau, blocked with % non-fat dry milk (Sigma Aldrich) for 1 h and incuated overnight at 4 ◦C with the following primary antibodes: anti-GFP (Thermo Fisher Scientific, A-11122, 1:1000), nti-TIAR (BD Transduction, 610352, 1:1000), anti-FUS SantaCruz Biotech, sc-47711, 1:2000), anti-GAPDH-HRP SantaCruz Biotech, sc-47724, 1:1000), anti-FLAG (Merck, 3165, 1:1000), anti-ELAVL1 (SantaCruz Biotech, sc-5261, :1000), anti-DHX36 (Proteintech, 13159–1-AP, 1:500) and nti- βActin (SantaCruz Biotech, sc-69879, 1:1000). .. After secndary antibody incubation for 1 h at room temperature (RT), rotein detection was carried out with Immobilon Crescendo estern HRP substrate (Merck) using ChemiDocTM MP Sysem and images were analyzed using Image LabTM Software Bio-Rad). urom ycilation assay US expression was induced in SK-N-BE cells as stated above. fter 1 h of stress with 0, 5 mM sodium arsenite, the medium as removed and cells were washed twice in warm medium. ells were then incubated in culture medium supplemented ith 10 μg / ml puromycin (Merck, P4512) for 0, 3 or 4 h. fter the incubation, cells were washed twice in phosphateuffered saline (PBS), then lysed in RIPA buffer and sonicated o optimize protein solubilization.



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    96
    Santa Cruz Biotechnology nti βactin
    Nti βactin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nti+%CE%B2actin/%CE%B2-Actin+Antibody/pm39494508-51-62-64
    Average 96 stars, based on 1 article reviews
    nti βactin - by Bioz Stars, 2026-10
    96/100 stars
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